| Description |
Microarrays have revolutionized genomic studies. Protein arrays have the same potential to impact proteomics. Protein arrays can be used to screen for interaction of proteins with other proteins, DNA, antibodies, cells, or small molecules. The two major formats designed to serve rather different applications present different technical challenges and opportunities. Immobilization of numerous proteins requires efficient protein expression and strategies that retain activity. Determination and quantification of protein expression levels require arrays designed to selectively capture proteins in solution. Numerous technical obstacles, such as optimizing protein immobilization or selection of protein-binding ligands, strategies for extending the limits of detection, and novel applications of this exciting technology, are among the issues that will be discussed in detail.
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