Event Date/Time: Mar 21, 2005
End Date/Time: Mar 22, 2005
Changes in gene expression underlie many biological phenomena. Quantitative PCR (qPCR) or real time PCR is a highly sensitive method that utilizes small sample sizes and short experimental time frames. qPCR is especially useful for evaluating “RNA phenotypes” obtained from microarray or siRNA experiments. These gene expression technologies generate vast amounts of data. Yet, there are often limited resources for subsequent independent validation studies. Cost, time, reagents, kit selection, and instrumentation considerations are crucial. Researchers, using microarrays or RNAi for mapping cellular pathways, prioritizing suitable targets for pharmaceutical intervention or diagnostics, determining toxicology or prognostics (disease progression), will find this meeting useful for developing qPCR as their validation tool of choice.