Real-time PCR hands-on workshop (QPCR)
Venue: Lundberg laboratory
|Event Date/Time: Aug 23, 2005||End Date/Time: Aug 25, 2005|
TATAA Biocenter Open Course in QPCR.
We have found that the demand for training in the field of
real-time PCR (QPCR) is huge and to coordinate these we aim to arrange three
day courses. The first course will be directed to people planning or considering
using QPCR in their research and also users not yet fully familiar will QPCR.
The second day targets more advanced users and people concentrating on different
quantification strategies. The third day focuses on aspecs in sample preparation
and reverse transcription. The courses contain both theoretical seminars and
practical hands-on training with experienced supervision. The price for attending
these courses are 2000 SEK per day and person.
The basic course consists of a theoretical part and a practical part where
participants get to do QPCR experiments by themselves under experienced supervision.
The course contains:
*Basic PCR theory
*The theory of real-time PCR
*Applications and possibilities of QPCR. Comparison of QPCR with regular PCR.
*Review of currently available detection technologies (SYBR Green I, TaqMan,
*Different instrument plattforms and their typical uses
*The problem of primerdimer fromation and how to minimize them
More advanced users will, apart from a brief review of the above, also learn
*Introduction to quantification principles
*Quantification strategies, uses and limitations
*Calculations using different relative quantification methods
*Strategies for normalization of QPCR data
*In situ calibration for compensation of inhibition in samples
This course covers aspects in sample preparation and reverse transcriptions.
*Principles of RT
*Priming methods for RT
*What enzymes are preferential for different applications
*Sample Preparation (Extraction of RNA and DNA)
*Other applications: Multiplexing and SNP analysis