Protein Purification: Principles and Practice (7/22/07) (Protein Purification)
Venue: Rutgers University
|Event Date/Time: Jul 22, 2007||End Date/Time: Jul 27, 2007|
This five and one half day laboratory course covers a wide variety of conventional methods for protein isolation, purification, and characterization. The course format integrates hands-on laboratory exercises with classroom lectures, demonstrations, study breaks, and short take-home assignments. This course uses the remarkable Green-Fluorescent Protein (GFP), a novel marker for gene expression,
as the source material. While this is a general course applicable to all proteins,
we use GFP as a highly effective visual teaching tool.
A special feature of the course is that all laboratory work will be performed on the same starting sample (Aequorea GFP or recombinant GFP), which will be purified from an exceedingly crude form (starting with tissue or bacterial cell extraction) to near homogeneity as judged by high performance liquid chromatography (HPLC), SDS gel electrophoresis, isoelectric focusing, and western blotting. This feature provides a continuity of purpose, integrating dozens of preparative and analytical protein techniques in a way that few competing courses can match.
A problem-solving approach will be used throughout the course. Under the guidance of experienced lab instructors, participants will work in groups of three to plan their own protocols, analyze data, and interpret results. A student-teacher ratio not greater than 8:1 will be maintained and the faculty coordinators will be present throughout the course.