“Intact Cell Mass Spectrometry for Microbe Identification and Recombinant Protein Analysis”
Live Web Seminar
Tuesday, August 14, 2007

Speaker:
Mr. Martin Winkler, PhD.
Consultant, The Biotech Advisor

Topic:
“Intact Cell Mass Spectrometry for Microbe Identification and Recombinant Protein Analysis”
• Intact Cell Mass Spectrometry (IC-MS): discovery and development
• MALDI-TOF mass spectrometry instrument and methods
• Analytical Variables: IC-MS
• Identification of bacteria by IC-MS
• Two examples of pathogenic bacteria: Campylobacter and H. pylori
• IC-MS for molecular biology and protein screening
• Example of recombinant HIV antigen analysis by IC-MS
• Mutant recombinant protein detection and the analysis of post-translational modifications
• Soluble vs. insoluble recombinant protein detection


Learning Objectives:

• Understand the means of mass spectrometry
• Become familiarized with the benefits of various mass spectrometry methods
• Learn about new solutions and instruments for mass spectrometry
• Gain in-depth knowledge of intact cell mass spectrometry for microbe identification and recombinant protein analysis

Who Should Attend: • Those interested in rapid detection of bacteria, spores, and viruses from culture and environment or as possible bio-threat agents will want to learn about new technologies. • Microbiologists, biological scientists, mass spectrometrists and other scientists will find these methods powerful yet straightforward in application for detection, identification and analysis of microorganisms. • Researchers using high-throughput screening for rapid selection and detection of bacterially or virally expressed proteins need to know about the available techniques. • Molecular biologists needing a more rapid, more powerful alternative to SDS-PAGE for screening and analysis of bacterial clones expressing recombinant proteins