VI Workshop Rapid methods and automation in food microbiology (Workshop MRAMA)

Venue: Lecture Hall, Faculty of Veterinary

Location: 08193 Bellaterra, Barcelona, Spain

Event Date/Time: Nov 20, 2007 End Date/Time: Nov 23, 2007
Registration Date: Nov 12, 2007
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Tuesday, 20th November
8:30-9:30 a.m. Handout material delivery
9:30-10:15 a.m. Opening lecture by Dr. Cécile Lahellec (Agence Française de Sécurité Sanitaire des Aliments –AFSSA–, Alfort, France):
• “Impact of the development of microbiological rapid methods on food safety implementation in the European Union”
10:15-11:30 a.m. Dr. Daniel Y. C. Fung:
• Introduction to rapid methods and automation
• Sampling and sample preparation of solid and liquid foods, surfaces, and air
• Miniaturization: microtiter plate, most probable number (MPN), viable cell counts, and so on. Diagnostic kits
11:30 a.m.-12 p.m. Break
12-1:30 p.m. Dr. D. Y. C. Fung:
• Methods for viable cell counts: hydrophobic grid, spiral plating, flow cytometry, direct epifluorescent filter technique (DEFT), and so on
• Other methods to detect microorganisms: Fung’s double tube, Lee tube, Fung-Yu tube, and so on.
1:30-3 p.m. Lunch
3-4 p.m. Dr. Armand Sánchez (Universitat Autònoma de Barcelona –UAB–, Bellaterra –Cerdanyola del Vallès, Barcelona–):
• “Polymerase chain reaction (PCR)”
4-5:15 p.m. Microbiology companies: multimedia
5:15-5:30 p.m. Break
5:30-6:30 p.m. Dr. Daniel Ramón (Instituto de Agroquímica y Tecnología de Alimentos –IATA–, CSIC, Burjassot, Valencia):
• “Food nutrigenetics, nutrigenomics and transgenic organisms”
6:30 p.m. Merienda

Wednesday, 21st November
9-10:20 a.m. Dr. D. Y. C. Fung:
• Methods for viable cell counts, based on electric impedance, conductance and capacitance
• Methods for viable cell counts, based on bioluminiscence (ATP analysis) and colorimetry
10:20-11:50 a.m. Microbiology companies: multimedia
11:50 a.m.-12:20 p.m. Break
12:20-1:45 p.m. Dr. D. Y. C. Fung:
• Immunological methods for microbial and toxin identification: immunomagnetic separation, ELISA and ELFA, lateral immunomigration, immunoprecipitation, latex agglutination, and so on
1:45-3:15 p.m. Lunch
3:15-4 p.m. Ms. Montse Vila (Central de Cocinados CATAR, S. A., Mollet del Vallès, Barcelona):
• “Application of predictive microbiology to food industry”
4-5:20 p.m. Microbiology companies: multimedia
5:20-5:35 p.m. Break
5:35-6:20 p.m. Dr. Rosa M. Pintó (Universitat de Barcelona, Barcelona):
• “Detection of foodborne viruses: prospects and limitations”
6:20 p.m. Merienda

Thursday, 22nd November
8:30-11:30 a.m. 1st laboratory session (mornings group)
8:30-11:30 a.m. Microbiology companies: live equipment and product exhibition
11:30-11:45 a.m. Break
11:45 a.m.-12:45 p.m. Dr. D. Y. C. Fung:
• Genetic methods for microbial identification: hybridization (DNA and RNAr probes); polymerase chain reaction (PCR); DNA characterization (fingerprinting, riboprinting); biosensors, biochips and microchips; proteomics; and so on
12:45-1 p.m. Official picture of the VI workshop MRAMA
1-2:10 p.m. 1st visit to Roche Diagnostics, S. L. facilities (Sant Cugat del Vallès, Barcelona), for applications of real-time PCR (limited attendance)
1-2:30 p.m. Lunch
2:30-3:15 p.m. Dr. Ferran Ribas (Societat General d’Aigües de Barcelona –AGBAR–, Barcelona):
• “Equivalence tests among methods of microbiological analysis: example of the Spanish test for coliforms and Escherichia coli”
3:15-4 p.m. Open discussion (with Dr. Fung, other lecturers, and people from microbiology companies) about instrumentation in food microbiology, world trade trends, and other current topics; with Dr. José Juan Rodríguez (Universitat Autònoma de Barcelona, Bellaterra –Cerdanyola del Vallès, Barcelona–) as chairman. Conclusions
4-4:30 p.m. Official adjournment of the workshop (celebration)
4:30-7:30 p.m. 1st laboratory session (afternoons group)
4:30-7:30 p.m. Microbiology companies: live equipment and product exhibition

Friday, 23rd November
9-11:30 a.m. 2nd laboratory session (mornings group)
10:15-11:45 a.m. 2nd visit to Roche Diagnostics, S. L. facilities (Sant Cugat del Vallès, Barcelona), for applications of real-time PCR (limited attendance)
11:45 a.m.-1:15 p.m. 3rd visit to Roche Diagnostics, S. L. facilities (Sant Cugat del Vallès, Barcelona), for applications of real-time PCR (limited attendance)
1:15-2:45 p.m. 4th visit to Roche Diagnostics, S. L. facilities (Sant Cugat del Vallès, Barcelona), for applications of real-time PCR (limited attendance)
3-5:30 p.m. 2nd laboratory session (afternoons group)


In these sessions, which will be given by Drs. Mª Manuela Hernández Herrero ( and Artur Xavier Roig Sagués (, different rapid methods will be assayed: Contact plates Mediasure. Air samplers: Sampl’air, MicroBio. Lyophilized microorganism strains. Cryobeads. Homogenizers: Pulsifier, Smasher. Dilumat and Dilubag. Dilucup / Dilushaker. Chromogenic and fluorogenic culture media: ASAP, SMS, ALOA, ODA, ESIA, Baird-Parker RPF, SM ID 2, COLI ID, O157:H7 ID, OAA, Count-Tact, OCLA, E. sakazakii medium, RAPID’E. coli 2, RAPID’L. mono, COMPASS L. mono, BD BBL CHROMagar Salmonella, BD BBL CHROMagar Listeria, BD BBL CHROMagar Staph aureus, BD BBL CHROMagar O157. Revolving plater. Colony counter. Spiral plater. Petrifilm (plates and reader). NEO-GRID. SimPlate. Diagnostic kits and readers: API, BD BBL Enterotube II, BD BBL Crystal ID system, RapID, O•B•I•S•, Microbact, Microgen ID. ATP – Bioluminiscence: luminometer systemSURE II (surface and rinse water control). Colorimetry: CLEAN TEST, PRO-Clean, SpotCheck (detection of glucose, lactose, protein residues). Immunology: immunomagnetic separation, ELISA (pathogens, mycotoxins, Veratox for histamine), Microscreen (latex agglutination), Reveal (pathogens and allergens), VIP, 1-2 Test. Others. Explanation about hands-free "Pop-up" adhesive tape sampling, Redigel, Fung’s double tube and Fung-Yu tube.
Attendance will be limited to 25 people per session.


People from companies will explain and show how their equipments and products work: Preparator, and automated pourer stacker of culture media. Petrifilm. LABGUARD monitor. Flow cytometry: BactiFlow. TEMPO (miniaturized and automated MPN). BiOLOG identification system. ATP – Bioluminiscence: luminometers ST12 and Promilite III (dairy sterility, fruit juice, wine, and soya test kits), Luminomat, Uni-Lite, AccuPoint, LIGHTNING MVP. Colorimetry: Soleris. Immunology: Dynabeads (immunomagnetic separation), VIDAS, TECRA UNIQUE PLUS. Real-time PCR (Taqman / RapidFinder, Warnex, Assurance GDS). Others.

Dr. Daniel Y. C. Fung ( is a professor of food science in the Department of Animal sciences and industry at Kansas State University (KSU), in Manhattan, Kansas (USA). His speciality is food microbiology, and he has gained an international reputation as a lecturer on rapid and miniaturized methods and automation. He has more than 800 publications in scientific journals, books and proceedings. Director of the annual international workshop on Rapid methods and automation in microbiology, which is held in Manhattan, KS and has reached the 27th edition. Recipient of the 1997 International Award given by the Institute of Food Technologists (IFT) because of the uniqueness of this workshop series, and winner of the 2001 Waksman Outstanding Educator Award given by the Society for Industrial Microbiology, the 2005 KSU College of Agriculture Excellence in Graduate Teaching Award, and the 2006 IFT Carl R. Fellers Award for a distinguished career in Food science and technology. Distinguished Professor of Veterinary College at UAB since 2006. On July 9, 2007, he received the Inaugural Outstanding Educator in Food Safety Award given by Food Safety magazine and ConAgra Foods, Inc. at the International Association for Food Protection (IAFP) national meeting (Orlando, Florida –USA–), for his teaching career with more than 18,000 students and 100 graduate students (33 Ph.D. and 67 M.S.) completed as the major professor. Editor of Journal of Rapid Methods and Automation in Microbiology. Fellow of the American Academy of Microbiology, the IFT and the International Academy of Food Science and Technology. He presented an invited lecture at Pasteur Institute in Paris (France) during the 100th year commemoration of Pasteur’s death in 1995.


Faculty of Veterinary. Edifici V. Universitat Autònoma de Barcelona Campus.

Additional Information

LANGUAGES English and Spanish Simultaneous translation will be provided FEES The registration fee for the laboratory sessions on 22nd and 23rd November is 40 €. Rest of the workshop: • UAB students: 12 € (plus 20 € if handout material is required) • UAB staff: 32 € • Non-UAB students: 120 € (or 60 €/day) • ACCA members: 180 € • “Alimentaria”; “Alimentación, Equipos y Tecnología”; or “Técnicas de Laboratorio” journal subscribers: 200 € • Others: 220 € (or 110 €/day) Students will have to prove their position. PAYMENT By bank transfer. The name of the bank is ”la Caixa”, and the number of the account is: IBAN-ES10-2100-0424-3702-0019-3047 BIC/CODIGO SWIFT CAIXESBBXXX. Include “VI workshop MRAMA”, and your first and last names. Send the receipt by fax. Registration fees will be refunded if notice of cancellation is received before 12th November. If you have questions concerning the workshop, contact: Josep Yuste ( Marta Capellas ( Phone: +34-93-5811446 Fax: +34-93-5811494