Protein Purification: Principles and Practice (Protein Purification)
|Event Date/Time: Mar 15, 2009||End Date/Time: Mar 20, 2009|
as the source material. While this is a general course applicable to all proteins,
we use GFP as a highly effective visual teaching tool.
A special feature of the course is that all laboratory work will be performed on the same starting sample (Aequorea GFP or recombinant GFP), which will be purified from an exceedingly crude form (starting with tissue or bacterial cell extraction) to near homogeneity as judged by high performance liquid chromatography (HPLC), SDS gel electrophoresis, isoelectric focusing, and western blotting. This feature provides a continuity of purpose, integrating dozens of preparative and analytical protein techniques in a way that few competing courses can match.
A problem-solving approach will be used throughout the course. Under the guidance of experienced lab instructors, participants will work in groups of three to plan their own protocols, analyze data, and interpret results.