Setting up an HCA Lab

Venue: San Francisco

Location: San Francisco, California, United States

Event Date/Time: Jan 05, 2009 End Date/Time: Jan 05, 2009
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Monday, January 5
Pre-Conference Advanced Workshop:
Setting up an HCA Lab
(Separate Registration Required)
8:30-9:00 Registration for the Advanced Workshop and Morning Coffee

9:00-10:30 Advanced Workshop Part I: Instrumentation and Automation
Anthony Davies, Ph.D., Director, High-Content Research Facility, Clinical Medicine, Trinity College, Ireland

1. Choosing the Right Platform for Your Facility: How to Avoid Making the Wrong Choice for the Right Reasons
Even the most experienced HCS users may have difficulty in choosing the right HCS platform for their research needs. This becomes an increasingly important issue when financial justifications have to be made for procurement. There are several High-Content Screening platforms currently available on the market. Choosing the right one for your research needs can often be difficult, especially when confronted by the wide choice of high quality options. In this workshop section we will outline an unbiased method for evaluating the suitability of four hypothetical high-content screening platforms, for a range of common research applications.

2. Strategies for Setting up HCS/HCA Labs
In this segment of the workshop we will examine the core issues involved in setting up an HCS/A laboratory. This involves examples of methods employed to organize personnel and training for the efficient running of the HCS lab. Finally, we will be looking into how to automate your HCS laboratory. We will explore the main classes of robotic instrumentation and their common uses in a variety of lab set-ups. The aim of these segments will be to provide the HCS user with the tools to develop their own long-term set-up strategy.

10:30-11:00 Networking Coffee Break

11:00-12:30 Advanced Workshop Part II: Assay Development
Ann F. Hoffman, Senior Principal Scientist, Roche Discovery Technologies

Ideal and Challenging Principals of HCA Assay Development
As high-content imaging platforms become a standard tool in both drug discovery and academic research efforts the necessity to develop “screenable” cell-based assays in short time periods has become a primary challenge. The development of both core and customized HCA assays covering experimental designs, methods in assay development and options in experimental practices and workflows and will be covered. This workshop section focuses upon key factors and questions that assist in developing both core and complex high-content screening assays.

12:30-1:30 Lunch

1:30-3:00 Advanced Workshop Part III: Data Analysis and Management
Karol Kozak, Ph.D., Head of Computation Analysis, HCA/HTS Informatics, LMC-RISC, Institute for Biochemistry

Practical Handling and Data Analysis of High-Content Screening Data
Cell-based high-content screening (HCS) using automated microscopy is an upcoming methodology for the investigation of cellular processes and their alteration by multiple chemical or genetic perturbations. The analysis of the large amount of data generated in HCS experiments represents a significant challenge and is currently a bottleneck in many screening projects. This workshop session reviews the different ways to analyse large sets of HCS data, including the questions that can be asked and the challenges in interpreting the measurements. The main data mining approaches used in HCS, such as image descriptors computations and classification algorithms, will be outlined.

3:00-3:30 Networking Refreshment Break

3:30-5:00 Advanced Workshop Part IV: Discussion with all Participants

5:00 Close of Day


For more information, please contact:
Julia Boguslavsky, Executive Director, Conferences

For sponsorship information, please contact:
Katelin Fitzgerald, Manager, Business Development
Phone: 781-972-5458