How to do Successful Gene Expression Analysis Using Real-Time PCR

Venue: San Diego

Location: San Diego, California, United States

Event Date/Time: Mar 15, 2009 End Date/Time: Mar 15, 2009
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Description

HOW TO DO SUCCESSFUL GENE EXPRESSION ANALYSIS USING REAL-TIME PCR
In this short course, all aspects of the real-time PCR work flow will be covered, including:

RNA quality control, assay design and validation, selection of stably expressed reference genes, and experiment design
Data analysis using state-of- the art strategies, methods, and quantification models
Importing quality controls during the work flow
Insights into state-of-the art normalization using multiple reference genes, error propagation, and inter-run calibration
An introduction to biostatistical analysis of gene expression
Who Should Attend & Why: Both the first time real-time PCR user and the seasoned experimenter will learn state-of-the-art strategies, algorithms and validated work flow for real-time PCR gene expression analysis will benefit from this course.

Course Instructors:
Jo Vandesompele, Ph.D., Professor, Center for Medical Genetics, Ghent University Hospital
Jan Hellemans, Ph.D., Center for Medical Genetics, Ghent University Hospital

*Separate Registration Required

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