EMBO Course on microRNA Profiling: From in-situ Hybridisation to New-Generation Sequencing (microRNA Profiling)
Venue: EMBL - european Molecular Biology Laboratory
Location: Heidelberg, Germany
Event Date/Time: Apr 18, 2009 | End Date/Time: Apr 24, 2009 |
Registration Date: Mar 01, 2009 |
Description
The course will start on Saturday afternoon with theoretical introduction into miRNA profiling. From Sunday onwards laboratory work and bioinformatics work will be interleaved with lectures, tutorials.
1. Hands-on laboratory work & accompanying tutorials:
- Quality assessment and quantification of total RNA;
- Set up of the complete microarray-microRNA profiling experiment;
- Set up of a complete qPCR experiment on various systems, reverse transcription and qPCR steps: synthesis of complementary DNA followed by microRNA specific amplification
- Set up of the complete in-situ hybridization experiment
- Set up of the complete bead-based microRNA profiling assay
- Data analysis by different algorithms, normalization approaches
- Correlation of miRNA expression data originating from various methods
- Troubleshooting of all individual steps
2. Lectures:
- Approaches to microRNA profiling (new-gen sequencing, bead-based, microarray, qPCR, Northern blotting,
- in-situ hybridization)
- Sample preparation
- Experimental design/setup
- Quality controls for total RNA
- Equipment/instrumentation options
- Design of qPCR assays for determination of miRNA expression
- Design of microarray microRNA experiment
- Data analysis, normalization and interpretation
- Correlation of transcriptome profiling with microRNA profiling data
- Correlation between various microRNA profiling approaches
- prediction of microRNA targets and their experimental confirmation
Teachers
Carsten Alsbo, Exiqon, Denmark
Dite Andreasen, Exiqon, Denmark
Foteini Christodoulou, EMBL Heidelberg, Germany
Anton Enright, EMBL-EBI, Cambridge, United Kingdom
David Ibberson, EMBL Heidelberg, Germany
Eric Miska, Wellcome Trust/ Cancer Institute, United Kingdom
Martina Muckenthaler, EMBL-MMPU Heidelberg, Germany
Tania Nolan, Sigma Aldrich, United Kingdom
Ramin Saheri, Luminex, USA
Thomas Sandmann, DKFZ Heidelberg, Germany
Keld Sorensen, Luminex, USA
Jens Stolte, EMBL Heidelberg, Germany
Wolfgang Wagner, Heidelberg University, Germany