EMBO Course on Fluorescene (Cross-) Correlation Spectroscopy (FCS/FCCS) for Cell Biology Application (Fluorescene (Cross-))
|Event Date/Time: May 18, 2009||End Date/Time: May 23, 2009|
|Registration Date: Mar 31, 2009|
Fluorescence fluctuation spectroscopy (also called fluorescence (cross-)correlation spectroscopy; FCS/FCCS) denotes a family of microscopy-based methods that allows to asses transport and mobility properties as well as interactions of molecules (protein-protein, protein-nucleic acid, protein-compound, protein complex formation) in vitro and more importantly, also in living cells.
Instruments for FCS/FCCS are commercially available, however, publications that use this method to address molecular interactions are sparse. The reason for this can be found in the complex data processing required to evaluate FCS/FCCS experiments. This knowledge is not commonly available in cell biology laboratories. However, recent software developments and standardizations of the instruments strongly suggest that FCS/FCCS will become a routine method in focused cell biological studies (but also for high content screening approaches), provided that experimental biologists have an opportunity to acquire the relevant knowledge. This requires in depth training on the subject that cannot be covered by more general microscopy courses.
The goal of this practical course is therefore to teach cell biologists how to use FCS/FCCS as a routine tool in order to investigate molecular interactions in living cells.
The course will start on Monday 18 May at 9:00 am and will end on Saturday 23 May at 7 pm.
We recommend arrival on Sunday May 17 when we will have a first get-together in the ISG hotel in the evening.
Jan Ellenberg, EMBL Heidelberg, Germany
Joerg Enderlein, Georg August University, GÃ¶ttingen, Germany
Masataka Kinjo, Hokkaido University, Sapporo, Japan
Karsten Rippe, DKFZ & Bioquant, Heidelberg, Germany
Petra Schwille, Technical University Dresden, Germany
Matthias Weiss, DKFZ & Bioquant, Heidelberg, Germany
Paul Wiseman, McGill University, Montreal, Canada
Thorsten Wohland, National University of Singapore, Singapore