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Aims
Fluorescence fluctuation spectroscopy (also called fluorescence (cross-)correlation spectroscopy; FCS/FCCS) denotes a family of microscopy-based methods that allows to asses transport and mobility properties as well as interactions of molecules (protein-protein, protein-nucleic acid, protein-compound, protein complex formation) in vitro and more importantly, also in living cells.
Instruments for FCS/FCCS are commercially available, however, publications that use this method to address molecular interactions are sparse. The reason for this can be found in the complex data processing required to evaluate FCS/FCCS experiments. This knowledge is not commonly available in cell biology laboratories. However, recent software developments and standardizations of the instruments strongly suggest that FCS/FCCS will become a routine method in focused cell biological studies (but also for high content screening approaches), provided that experimental biologists have an opportunity to acquire the relevant knowledge. This requires in depth training on the subject that cannot be covered by more general microscopy courses.
The goal of this practical course is therefore to teach cell biologists how to use FCS/FCCS as a routine tool in order to investigate molecular interactions in living cells.
Programme
The course will start on Monday 18 May at 9:00 am and will end on Saturday 23 May at 7 pm.
We recommend arrival on Sunday May 17 when we will have a first get-together in the ISG hotel in the evening.
Invited Speakers
Jan Ellenberg, EMBL Heidelberg, Germany
Joerg Enderlein, Georg August University, Göttingen, Germany
Masataka Kinjo, Hokkaido University, Sapporo, Japan
Karsten Rippe, DKFZ & Bioquant, Heidelberg, Germany
Petra Schwille, Technical University Dresden, Germany
Matthias Weiss, DKFZ & Bioquant, Heidelberg, Germany
Paul Wiseman, McGill University, Montreal, Canada
Thorsten Wohland, National University of Singapore, Singapore
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| Additional Information |
Registration
Please register online.
Deadline for registration is 31 March 2009.
Registration types and fees:
normal: EUR 400 including accommodation (7 nights) in a twin room, meals, local transport and course material.
industry: subject to inquiries.
Confirmation and Payment
The registration fee should not be paid before acceptance to the course.
Scientific Organisers
Philippe Bastiaens, MPI Dortmund, Germany
Michael Knop, EMBL, Heidelberg, Germany
Malte Wachsmuth, EMBL, Heidelberg, Germany
Hotel
Twin room accommodation is included (7 nights) in the participation fee for the default dates of May 18 - 23, 2009. Rooms have been reserved at the ISG Hotel.
Please enter your dates of arrival and departure as well as requests for specific room mates or single room accommodation via the registration link.
Single rooms will be provided on a first come, first served basis at extra cost (approx. EUR 25 per night). All additional nights (beyond the 7 nights twin room paid by EMBL) will be charged extra (approx. EUR 75 per night for single accommodation and EUR 100 for twin accommodation).
Local Transport
There will be shuttle buses running from the ISG hotel to EMBL and back mornings and evenings. A detailed schedule will be available closer to the event.
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