A joint EMBL/Agilent Technologies Practical Course on MicroRNA profiling (MicroRNA profiling)
|Event Date/Time: Jul 20, 2009||End Date/Time: Jul 24, 2009|
Agilentâ€™s microarray platform is built on the unique non-contact inkjet printing process generating in situ synthesised oligonucleotides. It allows for the usage of multiple applications such as miRNA profiling, gene expression analysis, CGH (comparative genomic hybridisation), ChIP-on-Chip (chromatin immunoprecipitation on microarrays), or DNA methylation studies.
MicroRNAs (miRNAs) are an abundant class of small single-stranded non-coding RNAs (19-30 nucleotides long) that serve widespread functions in post-transcriptional gene silencing.
Recently, research has demonstrated that miRNAs are crucial regulators of gene expression, affecting a wide variety of cellular functions including development, proliferation, differentiation, and apoptosis.
This workshop will combine both layers of information, miRNA profiling and gene expression, and provides an integrated analysis with the industry-standard GeneSpring software.
In this course we will go through all steps of a microarray-based miRNA and gene expression profiling experiment, starting from RNA QC using the Agilent Bioanalyzer through the labelling, hybridisation and scanning up to data analysis using GeneSpring GX.
- RNA QC with Agilent Bioanalyzer and Nanodrop
- labelling and purification
- Hybridisation of labelled targets
- Washing and scanning
- Data extraction and quality assessment
- Data analysis
- Introduction to the Agilent microarray platform
- Quality control of total RNA using the Bioanalyzer
- Data generation and data extraction with Feature Extraction software and GeneSpring GX
The programme for 2009 is not yet available.
Please refer to last year's programme Agilent 2008 for an impression.