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PCR’s sensitivity, specificity, and simplicity has revolutionized molecular biology in basic, industrial, and clinical settings. Technologies abound including quantitative PCR (qPCR), multiplex qPCR, real-time PCR, and reverse transcription quantitative PCR (RT-qPCR). Your technology selection is determined by the source of nucleic acid. However, complexities arise because of multiple assays, numerous protocols, abundant sources, lack of assay standardization, differences in sample processing, ineffective use of controls, normalization methods, and quality control management. Researchers face challenges in reliability, relevance, and reproducibility. Learn from savvy, seasoned researchers as they share their real-world experiences, applications, and results at Cambridge Healthtech Institute’s 5th Annual Quantitative PCR.
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