BIT Life science 2nd Annual World congress of industrial Biotechnology 2009 (ibio)

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Event Date/Time: Apr 05, 2009 End Date/Time: Apr 07, 2009
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1-initiation and maintenance of tissue cultures at two photoperiod. 2-Extraction of essential oil using steam distillation nethod from plant leaves and callus cultures. 3-Ethanolic extraction of leaves and callus cultures. 4-Identification and quantitation of essential oil in plant leaves and callus essential oil by using Gas chromatography-Mass spectrometry. 5. Comparison between the yield of essential oil in leaves and callus cultures



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The yield of Rosemary plant for some secondary metabolites,was investigated.Yield of tissue culture was compared with intact plant production.Many experiments were implemented.Callus was initiated on (MS)medium supplemented with combination of 0.0,0.1,0.5,and1.0mg/L BAand0.0,0.5,1.0,1.5,and2.0mg/L2,4-D.Maximum callus frish weight waqs obtained by the combination of 0.1mg/L BAand0.5mg/L2,4-D,under 16hrs light and 8hrs dark.Highest callus fresh weight initiated in full dark ocurred at 0.5mg/LBAand0.5mg/L of 2,4-D.Many combination were examined for callus maintenance.It was found that the best combination for callus maintenance under 16hrs light and 8hrs dark was 0.5mg/L BA 0.1mg/L2,4-D,where as maximum callus fresh weight was obtained in the absence of light when 0.5mg/L of both plant regulator were added to the culture medium.The plant content of essential oil was estimated monthly .Essential oil was at highes level during the winter season. The chemical analysis was carried out using (GC-MS).Theessential oil of rosemary leaves was characterized by a higher content of 8-pinene(21.0935%),camphor(12.889%),borneol99.808%),eucalyptol(8.257%) andcamphene(7.973%),while the main constituents of callus essential were camphor(19.961%),borneol(19.160%),thymol(8.497%) and eucalyptol(10.126%).