Laser Microdissection - A Day For Answers
Venue: The Biopark
Location: Welwyn Garden City, United Kingdom
Event Date/Time: Jun 17, 2010 | End Date/Time: Jun 17, 2010 |
Early Registration Date: Mar 20, 2010 | |
Abstract Submission Date: Mar 10, 2010 |
Description
Talks include
Laser Microdissection in Parasitology - Professor Rory Post - Department of Infectious and Tropical Diseases, London School of Hygiene & Tropical Medicine
Laser Capture Microdissection of Mouse Cortex - Mary Antypa - University College London
Automatic detection of spermatozoa for laser capture microdissection - Mado Vandewoestyne,, Laboratory of Pharmaceutical Biotechnology, Ghent University , Belgium
In sexual assault crimes, laser capture microdissection (LCM) enables separation of spermatozoa and epithelial cells. We describe an automated screening method to detect spermatozoa stained with Sperm HY-LITERTM, a fluorescent kit for the detection of human spermatozoa. Different ratios of spermatozoa and epithelial cells as well as real postcoital samples were used to assess the automatic detection method. Detected spermatozoa were isolated using LCM and DNA analysis was performed. Robust DNA profiles without allelic dropout could be obtained from as little as 30 spermatozoa recovered from postcoital samples, showing that the staining and LCM had no significant influence on DNA recovery.
Additional Speakers
Dr Roman Gonitel - Medical and Molecular Genetics, King's College London
The Deadline for abstract submissions for oral presentation is March 10th 2010. Abstracts for poster presentation only can be submitted up to two weeks before the event. Please note that there will be a best poster prize. All accepted abstracts will be published in the meeting proceedings
The Deadline for early registration is March 20th 2010. After this time the fees double, so make sure you register early.