Characteristics of an Effective Mammalian Cell Line Generation Process for Bio-Pharmaceutical Produc

Venue: Complimentary online conference

Location: Toronto, Canada

Event Date/Time: May 25, 2010 End Date/Time: May 25, 2010
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Quickly and consistently generating stable, high productivity cell lines that produce protein of outstanding quality are the goals of any mammalian cell line process.

This talk will explore how steps in the generation of mammalian cell lines were optimized to make a process more efficient and outline certain risks that were taken to improve timelines and throughput.

Data collected from the development of over 200 cell lines, each producing a different antibody or recombinant protein will be summarized. An outline of our current CHO cell line development process that consistently yields clonal lines and upstream culture conditions that result in 2.5 – 5.0 g/L antibody production in five to six months will be presented.

Discussed topics will include:

Optimizing the Gene Construct
Minimizing Resources and Time During Clonal Selection
Balancing Titer and Stability vs. Speed
Addressing Protein Quality and Other Characteristics During the Process
Finding a Quick Upstream Development Process that Works Consistently
Continuing Process Improvement