EMBO Practical Course - Methods in Chemical Biology (CHB11-01)

Venue: EMBL Advanced Training Center

Location: Heidelberg, Germany

Event Date/Time: Mar 27, 2011 End Date/Time: Apr 02, 2011
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We will encourage the participants to apply methods of this interdisciplinary field to answer their biological questions. Emphasis will be on chemical protein engineering methods, proteomics methods, screening techniques and in vivo protein labelling as well as enzyme activity probes. Thereby, emphasis will be on (commercial) availability of the methods and reagents and on understanding of the chemical methods. Participants will do hands-on experiments supervised by experts in the field and will have plenty of time to discuss their own research problems with teachers and invited speakers.

Topics that will be covered include:


In vivo protein labeling via Snap- and Clip-tag technology

Development and application of nucleic acid probes for detection of RNA in cells

Photocontrollable and small-molecule-switchable enzymes

Miniaturized lipid arrays for screening of protein-lipid interactions

Mass spectrometry and liquid chromatography for the identification of proteins and posttranslational modifications

Introduction of unnatural amino acids via amber suppression for site-specific protein labeling

Expressed Protein Ligation and native chemical ligation

Assay development and validation for high throughput screening in microtiter plate format

Microfluidic technologies for single-cell/single organism assays and high throughput screens


Meyerhofstr. 1