qPCR 2011 - 5th international qPCR Symposium & Industrial Exhibition & Application Workshop (qPCR 2011)

Venue: Technical University Munich, in Freising-Weihenstephan

Location: 85354 Freising, Bavaria, Germany

Event Date/Time: Mar 28, 2011 End Date/Time: Apr 01, 2011
Registration Date: Mar 28, 2011
Early Registration Date: Jan 31, 2011
Abstract Submission Date: Jan 31, 2011
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Description

The focus of the qPCR 2011 Event will be on:

Molecular Diagnostics: from single-cells to Next Generation Sequencing
Leading academic researchers and industrial contributors in the field will participate in the symposium, which will be an arena for fruitful discussions between researchers of different backgrounds. The Symposium Talks, Poster Sessions, Industrial Exhibition and associated qPCR Application Workshops offer an overview of the present knowledge and future developments in qPCR and gene expression measurement technology and its wide applications.

Symposium Talk and Poster session:

Main topic: Molecular diagnostics in single-cells
Single-cell sampling, circulating tumor cells (CTS), pre-amplification techniques, laser micro dissection, sub-cellular PCR, micro-manipulation of cell clusters, cellular micro injection, FACS spotting, single-cell handling, …
Markers in diagnostic, prognostic, and therapeutic, markers on DNA, RNA, microRNA level, disease markers, tissue specific markers, cancer markers, stem-cells markers, differentiation markers, methylation markers diagnostic quantification methods, epigenetics, SNP analysis, high resolution melt (HRM) applications, ...

Main topic: High throughput analysis in qPCR & NGS (Next Generation Sequencing)
Next generation Sequencing (NGS); high throughput and gene expression profiling platforms, 384 – 1536 well applications and more, NGS application on DNA, mRNA and microRNA level, comparison of NGS with conventional high throughput qPCR, qPCR robotics; high throughput SNP application, real-time RT-PCR arrays (mRNA and microRNA), quantitative multiplexing …

MIQE and QM strategies in qPCR
The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Following these guidelines will encourage better experimental practice, allowing more reliable and unequivocal interpretation of qPCR results. QM strategies in real-time PCR to guarantee better and more valid results...

small RNAs qPCR - microRNA and siRNA Applications
RNAi mechanism, microRNA extraction, qRT-PCR technologies to detect microRNA, siRNA applications in combination with qRT-PCR, microRNA targets and microRNA precursors, new siRNA manipulation and microRNA technologies, .....

Digital PCR & Nano-fluidics
Digital PCR (dPCR) can be used to directly quantify and clonally amplify nucleic acids including DNA, cDNA, mRNA or microRNA. It allows a more reliable collection and sensitive measurement of nucleic acid amounts, applications in copy number variants, point mutations, and molecular diagnostics; …

Pre-analytical Steps
Pre-amplification, sampling technologies, DNA / RNA purification, extraction efficiency, DNA / mRNA / microRNA quality control, Reverse Transcription, RT quality control, external references, ...

qPCR BioStatistics & BioInformatics
Software applications, data mining, calculation of relative expression, primer and probe design on mRNA and microRNA level, real-time PCR efficiency determination, mathematical modelling, multivariate expression profiling raw data analysis, statistics in real-time PCR, data management, multi-way expression profiling, multiple regression analysis, 3D data visualization.......

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