EMBL Advanced Course - Targeted Genome Editing using Zinc Finger Nucleases

Venue: EMBL Advanced Training Centre

Location: 60117 Heidelberg, Germany

Event Date/Time: May 10, 2011 End Date/Time: May 12, 2011
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ZFNs are a class of engineered DNA-binding proteins that facilitate targeted editing of the genome by creating double-strand breaks (DSBs) in DNA at user-specified locations. DSBs are important for site-specific mutagenesis in that they stimulate the cell’s natural DNA-repair processes, namely homologous recombination and non-homologous end joining. Using well-established and robust protocols, these cellular processes can be harnessed to generate precisely targeted genomic edits resulting in cell lines, including somatic cell lines, with targeted gene deletions (knockouts), integrations, or modifications. Featured lectures will provide in depth discussion on the fundamentals of ZFNs as well as cutting edge applications for targeted gene knockout, integration and tagging. Additionally, each of the participants will conduct laboratory exercises to become proficient in various techniques for ZFN delivery, identification of mutants, and methods for quantitating ZFN efficiency. Registrants should be familiar with basic mammalian cell culture techniques, PCR and clonin


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