qPCR Experience® Boot Camp, Piran, Slovenia, 11.-14. September 2012 (qPCRBootCamp2012)

Venue: Marine Biology Station Piran

Location: Piran, Slovenia

Event Date/Time: Sep 11, 2012 End Date/Time: Sep 14, 2012
Registration Date: Sep 01, 2012
Early Registration Date: Jul 27, 2012
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qPCR Experience Boot camp will focus on real-time PCR gene expression and microorganism detection but will also cover qPCR's other applications such as single cell qPCR, digital PCR and will include extensive practical sessions and a troubleshooting* section.

Real-time PCR technology is an essential technique in every molecular biology lab for many reasons: impecable sensitivity, accuracy and scalability to high-throughput. It is therefore applied in various areas of life sciences, from pathogen or GMO determination and quantification, gene expression studies, genotyping etc.

If you haven't mastered it yet, here's your chance. The hands-on 4-day Boot Camp is suitable also for qPCR beginners and will cover review of qPCR theory, practical guidance on sample preparation and RNA quality control, amplicon design, quality assurance as well as hands-on lab and data analysis sessions. Instructors, all experienced researchers and users of qPCR technology, will also be available for discussions. Boot Camp will also host Dr. Stephen A. Bustin as a guest lecturer.

The workshop is organised in collaboration with National Institute of Biology. The lectures and dry-lab hands-on sessions will take place at the Marine Biological Station (MBS) in Piran, situated directly on the Mediterranean coast. The wet-lab sessions will take place in ISO accredited laboratories of National Institute of Biology in Ljubljana which include state-of-the-art qPCR centre.

All participants of last year's qPCR Experience Boot Camp would recommend it to others!

So join us in September on our second Boot Camp and tame the knowledge of real-time PCR in a lovely, relaxing and energetic environment of an old coastal town of Piran, a pearl of Slovenian coast.

*Troubleshooting section: to get the mot of the workshop we encourage you to send us your questions, remarks, special areas of interest, problems, data, etc. on which you would lilke extra information or concrete answers. We will do our best to give you answers already during the workshop or at special Troubleshooting section. Please sent the information until 28th August.


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Additional Information

Workshop programme Tuesday, September 11 (3 pm - 7 pm) • Arrival & registration • Real-time PCR principles and applications • Review of real-time PCR chemistries and equipment • Experiment setup - gene expression • Experiment setup - detection of microorganisms (diagnostic aproach) • Get together & relaxed walk-through Piran, night photosession Wednesday, September 12 (8 am - 6:30 pm) • Bus to ISO accredited lab (NIB, Ljubljana) where practical work will be held in groups • qPCR Validation (by S.A. Bustin) • Intro to workflow in Quality assurance compliant laboratory • Sample preparation & possibilities of automation • RNA quality control with Bioanalyzer • Reaction and qPCR run setup • Guest lecture by Stephen Bustin "Scientific integrity: can Molecular Medicine be trusted?" where the caution needed when interpreting qPCR results will be discussed (lecture is open to public) • Walk through Ljubljana city centre, dinner at castle and bus trip to Piran Thursday, September 13 (9 am - 5:30 pm) • Primer and probe design & Validation- principles (by S.A. Bustin) • Introduction to data analysis: SDS and melting curve analysis • Relative quantification strategies and normalisation • Practical data analysis of real gene expression data • Tour on the coast (details still to come) Friday, September 14 (9 am - 1 pm) • Practical data analysis of real diagnostic data • MIQE guidelines (minimum information for publication of quantitative real-time PCR experiments) • Single-cell real time PCR • Troubleshooting • Wrap-up and discussion